In Vitro Study of Anti-cancer Properties of Egyptian Scorpion (Leiurus quinquestriatus) Venom on Triple Negative Human Breast Cancer Cell Line MDA‑ MB‑ 231

Document Type : Original Article

Authors

1 Department of zoology, Faculty of science, Zagazig university, Egypt

2 Department of Zoology, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.

3 Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, 6th of October City, Giza 12578, Egypt

Abstract

Despite advances in therapy of breast cancer, it still remains a significant health concern. During the past recent years, various research studies have encompassed that novel therapeutic approaches are urgently needed. Scorpion venom has emerged as a promising anti-cancer agent. This is achieved primarily via scorpion venom-derived peptides revealed high and selective affinity for ionic channels over-expressed in many cancer cells. In particular, This study refer to the anti-cancer properties of L. quinquestriatus venom against human breast cancer cells MDA-MB-231. Cell viability was assessed using MTT assay. Apoptosis induction was evaluated using Annexin V/PI staining kit. Wound healing assay was employed to analyze cell migration. The MTT assay results demonstrated that L. quinquestriatus venom decreased the cell viability and had a selectively cytotoxicity against cancer cells, owing to its possible anti-cancer activity. The Annexin V-PI staining revealed a substantial increase in the percentage of apoptotic cells upon L. quinquestriatus venom treatment, and the wound healing assay showed a marked reduction by (~70%) in the migration ability after L. quinquestriatus venom treatment in comparison with non-treated cells. Significant decrease in human breast cancer cell viability (~ 60%) was observed after 48 hrs. of treatment with L. quinquestriatus venom compared with non-treated cells (P < 0.0001). These present findings provide evidence that L. quinquestriatus venom possesses potent anti-cancer properties and may be useful in the development of breast cancer treatment strategies.

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Bulletin of Faculty of Science, Zagazig University
Volume 2024, Issue 4
January 2025
Pages 108-118

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