the Potential cytotoxicity and preventive effect of Ferula Assafoetida against early carcinogenic changes in colorectal cancer

Document Type : Original Article

Authors

1 Department physiology and Zoology . Faculty of Science, Zagazig University

2 Department physiology and Zoology . Faculty of Science, Zagazig University, Egypt. 44519, Egypt

3 Medical Biochemistry and molecular biology, Cancer Biology Department, National Cancer Institute, Cairo University

4 Medical Biochemistry and Molecular Biology, Cancer Biology Department, National Cancer Institute, Cairo University,Egypt.

Abstract

preventive and cytotoxic effect of Ferula Assafoetida against early carcinogenic changes in colorectal cancer.
Methods: Trypain blue and MTT assay to measure the cytotoxicity and to evaluate the chemopreventive potential of Ferula Assafoetida on colorectal carcinoma cell line , using different Biochemical and Molecular technology .
Results: The results revealed dose dependent inhibition of the viability of Caco- 2cell line, with (IC-50) 342.9 μg/mL. The Ferula Assafoetida treated cells showed significantly, increase caspase-3,8,9 expression, decreased TNF-α expression compared to control. Studies carried out by T.A, GST and MDA were chosen for investigating antioxidant effect as additional mechanism for the chemoprevention explained that a balance between free radicals and antioxidants is necessary for proper physiological function . The inhibitory effect of Ferula Assafoetida on TNF-α as target protein and elucidate its binding site.
Conclusion: Ferula Assafoetida is a strong biological activity , preventive effect against cancer colon and a promising anticancer activity.
Keywords : Ferula Assafoetida , chemoprevention , cytotoxicity and Molecular Bioactivity.

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Bulletin of Faculty of Science, Zagazig University
Volume 2025, Issue 4
October 2025
Pages 144-160

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