Ecofriendly spectrophotometric techniques for the determination of imeglimin hydrochloride in pharmaceutical formulations

Gouda, Ayman A; Elsheikh, Ragaa; Abdelhamed, Asmaa S; Afify, Mohamed A.; El-Attar, Mona A; Almasry, Mahmoud

doi:10.21608/bfszu.2025.372235.1495

Ecofriendly spectrophotometric techniques for the determination of imeglimin hydrochloride in pharmaceutical formulations

Document Type : Original Article

Authors

¹ Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt

² Chemistry Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.

³ High Institute of Engineering & Technology, Tanta

10.21608/bfszu.2025.372235.1495

Abstract

Accurate, precise, eco-friendly, sensitive, and validated spectrophotometric methods were described for determination of imeglimin hydrochloride (IMG) in pure form and pharmaceutical formulations. These methods utilize N-bromosuccinimide (NBS) as an environmentally friendly oxidizing agent alongside three specific dyes: indigocarmine (IC), rhodamine B (RB), and orange G (OG). Under acidic conditions, excess NBS is used to oxidize IMG, and the unreacted NBS is subsequently quantified through its reaction with predetermined amounts of the dyes. Absorption measurements were recorded at 610 nm for IC, 550 nm for RB, and 478 nm for OG. The analytical technique was implemented and validated by carefully examining and optimizing various factors that could potentially affect the reaction. Strong linear correlations, with correlation coefficients ranging from 0.9990 to 0.9994, were established under optimal conditions. These methods were effective within concentration ranges of 1.0–14, 1.0–12, and 1.0–16 µg/ml, respectively. The limit of detection (LOD) was determined to be 0.29 µg/ml for IC, while RB and OG exhibited an LOD of 0.30 µg/ml. The accuracy and precision of these methods were assessed through both intra-day and inter-day measurements. No significant interference was detected from common tablet excipients.

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