Isolation and identification of urinary tract infection bacteria

Document Type : Original Article

Authors

1 Microbiology, Faculty of Science, Zagazig university

2 Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, EGYPT

3 Botany and microbiology depart.zagazig university

Abstract

Urinary tract infections (UTIs) are common infections caused predominantly by uropathogenic Escherichia coli (UPEC) bacteria, which cause approximately 80% of UTIs. A UTI is defined as significant bacterial growth of a single pathogen, at least 10,000 colony-forming units (CFU)/mL in clean-catch midstream urine. The healthcare sector continues to face a challenge in developing a drug that can simultaneously act on multiple pathogenic microorganisms. This study was designed to determine the prevalence of multi-drug-resistant bacteria (MDR) isolated from urinary tract infection (UTI) patients. A total of 100 isolates from urine samples were collected from hospitalized patients (30 males and 70 females) and were identified using conventional microbiological methods. The present study concluded that the isolated bacterial species were Acinetobacter spp., Klebsiella pneumoniae, and E. coli. were defined by common laboratory biochemical tests and confirmed by PCR technique. This isolated bacteria had an important role in urinary tract infections and most of them were multi-drug resistant.

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