Survey study on biofilm-forming bacteria contaminating Egyptian food products and controlling biofilm formation using natural products

Document Type : Original Article

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Zagazig University, Faculty of Science

Abstract

Forty food-borne pathogens, 36 (90%) Gram-negative and 4 (10%) Gram-positive bacteria, were isolated from ten food samples. All the food-borne pathogenic isolates were assayed for their biofilm production and the Bacillus cereus SR1 isolate exhibited the highest biofilm formation. Thirty-six lactic acid bacteria (LAB) were isolated from different Egyptian dairy products and screened for their antimicrobial activities against the selected isolate, Bacillus cereus SR1. The most potent strain giving strong antibacterial and antibiofilm activity was identified as Lactobacillus pentosus SR2. Therefore, it was chosen as the most active isolate against Bacillus cereus SR1 for this study. Both Bacillus cereus RS1 and Lactobacillus pentosus RS2 were identified by 16S r RNA and their sequences were submitted to the GenBank database under the accession numbers OP278950 and OP278963, respectively. In addition, six essential oils were tested for their antimicrobial activities against B. cereus RS1. It was found that the essential orange oil (EOO) was the most effective and exhibited higher antimicrobial activity against the tested strain. Therefore, the EOO was selected for our study. We concluded from this study that the essential orange oil and L. pentosus can be used as natural agents against bacterial growth and biofilm formation by food-borne bacteria.

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