Isolation and Characterization of v-KpPh: A Myovirus with Antimicrobial Potential against MDR Klebsiella pneumoniae

Document Type : Original Article

Authors

1 Department of Microbiology and Botany, Faculty of Science, Zagazig University, 44519, Zagazig, Egypt.

2 Department of Botany and Microbiology, Faculty of Science, Zagazig University, Egypt

3 Botany and microbiology department,Faculty of science,Zagazig University

Abstract

This study focused on isolating, characterizing, and assessing the bacteriophage v-KpPh for its ability to target and eliminates multidrug-resistant (MDR) Klebsiella pneumoniae and its biofilm. The v-KpPh phage displayed typical Myovirus morphology, with virion particles featuring a hexagonal icosahedral head measuring 90.34 nm in diameter, a narrowed neck region of 14.60 nm, and a long tail measuring 116.34 nm in length and 21.68 nm in width. Analysis of the phage’s biological traits revealed a latent period of 25 minutes, with phage release occurring at 35 minutes and a burst size of 51 pfu per cfu. Adsorption studies indicated that the v-KpPh phage attached to host cells within 15 minutes, with an adsorption rate constant (K) of 3.37×10⁻¹¹. The phage demonstrated stability across a pH range of 4 to 11 and at temperatures between 30°C and 60°C, retaining viability for up to 8 weeks when stored at room temperature or 4°C. Additionally, at a multiplicity of infection (MOI) of 10, the phage effectively reduced biofilm biomass, indicating its capability to eliminate both MDR K. pneumoniae and its biofilm formations.

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