Saqr, E., eldidamony, G., Askora, A. (2025). Isolation and Characterization of v-KpPh: A Myovirus with Antimicrobial Potential against MDR Klebsiella pneumoniae. Bulletin of Faculty of Science, Zagazig University, 2025(3), 17-37. doi: 10.21608/bfszu.2024.331414.1436
Ebtsam Saqr; gamal eldidamony; Ahmed Askora. "Isolation and Characterization of v-KpPh: A Myovirus with Antimicrobial Potential against MDR Klebsiella pneumoniae". Bulletin of Faculty of Science, Zagazig University, 2025, 3, 2025, 17-37. doi: 10.21608/bfszu.2024.331414.1436
Saqr, E., eldidamony, G., Askora, A. (2025). 'Isolation and Characterization of v-KpPh: A Myovirus with Antimicrobial Potential against MDR Klebsiella pneumoniae', Bulletin of Faculty of Science, Zagazig University, 2025(3), pp. 17-37. doi: 10.21608/bfszu.2024.331414.1436
Saqr, E., eldidamony, G., Askora, A. Isolation and Characterization of v-KpPh: A Myovirus with Antimicrobial Potential against MDR Klebsiella pneumoniae. Bulletin of Faculty of Science, Zagazig University, 2025; 2025(3): 17-37. doi: 10.21608/bfszu.2024.331414.1436
Isolation and Characterization of v-KpPh: A Myovirus with Antimicrobial Potential against MDR Klebsiella pneumoniae
1Department of Microbiology and Botany, Faculty of Science, Zagazig University, 44519, Zagazig, Egypt.
2Department of Botany and Microbiology, Faculty of Science, Zagazig University, Egypt
3Botany and microbiology department,Faculty of science,Zagazig University
Abstract
This study focused on isolating, characterizing, and assessing the bacteriophage v-KpPh for its ability to target and eliminates multidrug-resistant (MDR) Klebsiella pneumoniae and its biofilm. The v-KpPh phage displayed typical Myovirus morphology, with virion particles featuring a hexagonal icosahedral head measuring 90.34 nm in diameter, a narrowed neck region of 14.60 nm, and a long tail measuring 116.34 nm in length and 21.68 nm in width. Analysis of the phage’s biological traits revealed a latent period of 25 minutes, with phage release occurring at 35 minutes and a burst size of 51 pfu per cfu. Adsorption studies indicated that the v-KpPh phage attached to host cells within 15 minutes, with an adsorption rate constant (K) of 3.37×10⁻¹¹. The phage demonstrated stability across a pH range of 4 to 11 and at temperatures between 30°C and 60°C, retaining viability for up to 8 weeks when stored at room temperature or 4°C. Additionally, at a multiplicity of infection (MOI) of 10, the phage effectively reduced biofilm biomass, indicating its capability to eliminate both MDR K. pneumoniae and its biofilm formations.
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