Document Type : Original Article
Authors
1
Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt
2
Chemistry Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
3
Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
4
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
5
Chemistry Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt
Abstract
Glycerin is used in different concentrations according to the intended use such as an antimicrobial preservative, emollient, aqueous and non-aqueous gel vehicle, humectant, and plasticizer in tablet film coating and sweetening agent in alcoholic elixirs. A precise, accurate, and reversed-phase high-performance liquid chromatographic (HPLC) method was developed for the determination of glycerin in different pharmaceutical preparations. Analysis was performed on Microsorb-MV 100-5 NH2 (150 x 4.6 mm) or equivalent column with an isocratic mobile phase consisting of hexane buffer 0.005M (pH 3), Acetonitrile, and Methanol (12:78:10 v/v/v) at a flow rate of 1.0 mL/min and ambient temperature using photodiode array (PDA) detector at 202 nm. The retention time of the drug was found to be 3.011 ± 0.007 min. The injection volume was 20 µL. The mobile phase was used as a diluent during the standard and test sample preparation. The developed method was found to be linear over the range of 3.75-22.5 mg/mL for glycerin. The currently developed method can be easily performed in quality control laboratories for simultaneous determination of glycerin, where no expensive tools are available.
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