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Gouda, A., El Sheikh, R., Abdel Hay, S., El Sayed, H., Ali, M. (2023). Determination of glycerin in pharmaceutical formulations by liquid chromatography. Bulletin of Faculty of Science, Zagazig University, 2023(1), 153-163. doi: 10.21608/bfszu.2022.169206.1192
Ayman A Gouda; Ragaa El Sheikh; Soad S Abdel Hay; Heba El Sayed; Mohamedd Ali. "Determination of glycerin in pharmaceutical formulations by liquid chromatography". Bulletin of Faculty of Science, Zagazig University, 2023, 1, 2023, 153-163. doi: 10.21608/bfszu.2022.169206.1192
Gouda, A., El Sheikh, R., Abdel Hay, S., El Sayed, H., Ali, M. (2023). 'Determination of glycerin in pharmaceutical formulations by liquid chromatography', Bulletin of Faculty of Science, Zagazig University, 2023(1), pp. 153-163. doi: 10.21608/bfszu.2022.169206.1192
Gouda, A., El Sheikh, R., Abdel Hay, S., El Sayed, H., Ali, M. Determination of glycerin in pharmaceutical formulations by liquid chromatography. Bulletin of Faculty of Science, Zagazig University, 2023; 2023(1): 153-163. doi: 10.21608/bfszu.2022.169206.1192

Determination of glycerin in pharmaceutical formulations by liquid chromatography

Article 13, Volume 2023, Issue 1, March 2023, Page 153-163  XML PDF (1.27 MB)
Document Type: Original Article
DOI: 10.21608/bfszu.2022.169206.1192
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Authors
Ayman A Gouda email 1; Ragaa El Sheikh2; Soad S Abdel Hay3; Heba El Sayed4; Mohamedd Ali5
1Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt
2Chemistry Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
3Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
4Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
5Chemistry Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt
Abstract
Glycerin is used in different concentrations according to the intended use such as an antimicrobial preservative, emollient, aqueous and non-aqueous gel vehicle, humectant, and plasticizer in tablet film coating and sweetening agent in alcoholic elixirs. A precise, accurate, and reversed-phase high-performance liquid chromatographic (HPLC) method was developed for the determination of glycerin in different pharmaceutical preparations. Analysis was performed on Microsorb-MV 100-5 NH2 (150 x 4.6 mm) or equivalent column with an isocratic mobile phase consisting of hexane buffer 0.005M (pH 3), Acetonitrile, and Methanol (12:78:10 v/v/v) at a flow rate of 1.0 mL/min and ambient temperature using photodiode array (PDA) detector at 202 nm. The retention time of the drug was found to be 3.011 ± 0.007 min. The injection volume was 20 µL. The mobile phase was used as a diluent during the standard and test sample preparation. The developed method was found to be linear over the range of 3.75-22.5 mg/mL for glycerin. The currently developed method can be easily performed in quality control laboratories for simultaneous determination of glycerin, where no expensive tools are available.
Keywords
Glycerin; Reversed phase-HPLC; Method validation; Pharmaceutical preparations
Main Subjects
Basic and applied research of Chemistry,
Statistics
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